Staff Members - Academic

| Biological Sciences Staff | Publications |

Professor Roy Daniel

Role: Professor Qualifications: BSc [Hons] PhD Leic FRSNZ FNZIC Email: r.daniel@waikato.ac.nz Location: TRU.G.24 Contact: [+64] (7) 838 4213

Research Interests:

Current research is centered on the biochemistry, microbiology, and biotechnological applications of extremely thermophilic bacteria. Within these areas, major current topics are:
1) The inter-relationship of enzyme activity, stability, and dynamics;
2) The effect of temperature on enzymes;
3) Properties and applications of stable enzymes, especially proteases;

Research and postgraduate study are encouraged in the Department of Biological Sciences. All research students acquire basic research skills and a knowledge of techniques, as well as training in specialist disciplines.

See current graduate students.

See list of publications - [compiled by the Department of Biological Sciences].

Selected Publications:

Some Recent Publications:

Michelle E Peterson, Robert Eisenthal, Michael J Danson, Alastair Spence and Roy M Daniel. A new, intrinsic, thermal parameter for enzymes reveals true temperature optima. J Biol Chem, 279 (2004) 20717-10722.

Torsten Becker, Jennifer Hayward, Roy M Daniel, John Finney, Jeremy C Smith. Neutron frequency windows and the protein dynamical transition. Biophysical Journal. [In Press].

Dunn, R V, and Daniel, R M. The use of gas phase substrates to study enzyme catalysis at low hydration. Philosophical Transactions of the Royal Society of London Series B. [In Press].

Erika Balog, Torsten Becker, Martin Oettl, Ruep Lechner, Roy Daniel, John Finney and Jeremy C Smith. Direct Determination of Vibrational Density of States Change on Ligand Binding to a Protein. Rhys Rev Lett. [In Press].

Jennifer Hayward, John Finney, Roy Daniel, Jeremy Smith. Molecular dynamics decomposition of temperature-dependent elastic neutron scattering by a protein solution. Biophys J . 85, 679-685, 2003.

Jennifer Hayward, Roy M Daniel, John L Finney, Jeremy C Smith. Use of computer simulation in the interpretation of dynamic elastic neutron scatting in complex molecular systems: a small protein in various environments. Chemical Physics. 292 (2003) 389-396.

R.M. Daniel, R.V. Dunn, J.L. Finney, J.C. Smith. The Role of Dynamics in Enzyme Activity; Ann Rev Biophys Biomol Struct 32(2003) 69-92.

R.M. Daniel, J.F. Holden, R. van Eckert, J. Truter and D.A. Cowan. The stability of biomolecules and the implications for life at high temperatures. In Subsurface Biosphere at Mid-Ocean Ridges (W.S.D. Wilcock, C. Cary, E. DeLong, D.S. Kelley, and J.A. Baross, eds. American Geophysical Union Monograph, Washington, D.C.

James F. Holden, and Roy M. Daniel. The Upper Temperature Limit for Life Based on Hyperthermophile Culture Experiments and Field Observations. In Subsurface Biosphere at Mid-Ocean Ridges (W.S.D. Wilcock, C. Cary, E. DeLong, D.S. Kelley, and J.A. Baross, eds. American Geophysical Union Monograph, Washington, D.C (in press).

Lin Chen, Tim Coolbear and Roy M. Daniel. Characteristics of Proteinases and Lipases Produced by Seven Bacillus sp. Isolated from a Milk Powder Production Line. Int Dairy J 13 (2003) 255-275.

Roy M. Daniel: Astroenzymology - the environmental limits of enzyme activity, in Astronomical Telescopes and Instrumental 2002: Topics in Astronomy: Information Technologies, MMW and Sub-MMW Detectors, Solar Astrophysics, Non-EM Astronomy, Exo-Planet Detection and Astrobiology. The International Society for Optical Engineering, March 2003; ISBN 0-8194-4674-2.

L. Chen, R.M. Daniel and T. Coolbear. Detection and impact of protease and lipase activities in milk and milk powders: A review. International Dairy Journal 13 (2003) 255-275.

Roy M. Daniel, John L. Finney and Jeremy C. Smith. The dynamic transition in proteins may have a simple explanation. Faraday Discuss 122 (2002) 163-169.

R.M. Daniel, M.J. Danson and R. Eisenthal. The temperature optima of enzymes; a new perspective on an old phenomenon. Trends Biochem. Sci 26 (2001) 223-225.

P.L. Bergquist, M.D. Gibbs, D.D. Morris, A.M. Uhl, D.R. Thompson and R.M. Daniel. Hyperthermophilic xylanases. Methods in Enzymology, 330 (2001) 301-308.

R.M. Daniel and M.J. Danson. Assaying activity and assessing thermostability of hyperthermophilic enzymes. Methods in Enzymology, 334 (2001) 283-293.

Bragger, J.M., Dunn, R.V., and Daniel, R.M. Enzyme activity down to -100'C. Biochim. Biophys. Acta., 1480 (2000) 278-282.

Reat, V., Dunn, R., Ferrand, M., Finney, J.L., Daniel, R.M. and Smith, J.C. Solvent dependance of dynamical transitions in protein solutions. Proc Natl. Acad. Sci. 97 (2000) 9961-9966.

Toogood, H.S., Smith, C.A., baker, E.N. and Daniel, R.M. Purification and characterisation of AK.1 protease, a thermostable subtilisin with a disulphide bond in the substrate-binding cleft. Biochem. J., 350 (2000) 321-328.

Dunn, R.V., Reat, V., Finney, J.L., Ferrand, M., Smith, J.C. and Daniel, R.M. Enzyme activity and dynamics: Xylanase activity in the absence of fast anharmonic dynamics. Biochem. J., 346 (2000) 355-358.

Research Publications:

You may like to view publications from 1997 onwards for Professor Roy Daniel